PCR and microarray analysis of AmpC and ESBLs producing Pseudomonas aeruginosa isolates from intensive care units

Detection of AmpC and ESBL producing P. aeruginosa by phenotypic methods is challenging, especially in low-income countries such as Pakistan. Therefore, a molecular method was developed for rapid detection these resistance markers. A total 303 clinical samples were collected from intensive care units (ICUs) the Jinnah postgraduate medical centre (JPMC) Karachi, The isolates identified traditional matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF-MS). Isolates phenotypically analyzed AmpCs D-test double disc synergy, respectively. Check MDR CT103 XL PCR techniques used ESBLs. Out isolates, 148 (48.8%) aeruginosa. pattern against piperacillin, cefatizidime cefepime 59.4%, 64.8% 59.4% More than 60% resistant to aminoglycosides ciprofloxacin. All (148) strains found sensitive colistin. Phenotypic prevalence 8.8% whereas genotypic 29.1%. blaVEB most prevalent ESBL. Although 25.67% positive AmpC, microarray (Check-MDR) analysis did not detect chromosomally located any isolates.